![]() Picard is supported through the GATK Forums. See the Tool Documentation for details on the Picard command syntax and standard options as well as a complete list of tools with usage recommendations, options, and example commands. Java jvm-args -jar picard.jar PicardToolName OPTION1=value1 OPTION2=value2. The tools, which are all listed further below, are invoked as follows: ![]() If you don't, read on to the section on Getting Help. You should see a complete list of all the tools in the Picard toolkit. Or the environment variable that you set up as a shortcut (here we are using $PICARD): java -jar $PICARD -h To test that you can run Picard tools, run the following command in your terminal application, providing either the full path to the picard.jar file: java -jar /path/to/picard.jar -h If not, you may need to update your version see the Oracle Java website to download the latest JDK. ![]() If the output looks something like java version "1.8.x", you are good to go. To check your java version by open your terminal application and run the following command: java -version Unlike C-compiled programs such as Samtools, Picard cannot simply be added to your PATH, so we recommend setting up an environment variable to act as a shortcut.įor the tools to run properly, you must have Java 1.8 installed. Open the downloaded package and place the folder containing the jar file in a convenient directory on your hard drive (or server). The file name will be of the format picard-tools-x.y.z.zip. You can download a zipped package containing the jar file from the Latest Release project page on Github. The Picard command-line tools are provided as a single executable jar file. The Picard toolkit is open-source under the MIT license and free for all uses. For developers, the source code, building instructions and implementation/development resources are available on GitHub. Note that the information on this page is targeted at end-users. See especially the SAM specification and the VCF specification. These file formats are defined in the Hts-specs repository. Picard is a set of command line tools for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF. ![]() View the Project on GitHub broadinstitute/picard on a desktop it’s ok but on a laptop that might not always be connected to your local network, it’s probably better to mount them manually).A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF. ![]() Of course, they would have to be always accessible (i.e. If you have to easily access your network shares on your session, it might be a good idea to mount them at system startup in the directory you want through the fstab, rather than mounting them manually every time. Note that the 1000 in the path is the id of the first user created on Ubuntu: if you have multiple users and you’re not on the first account, it will be a different number. If it’s not the case with some, you would find them in /run/user/1000/gvfs, where you’ll see a folder with a name like “smb-share:server=xxx,share=yyy” (where xxx is the name of the server and yyy the name of the share). In some of the file open dialogs used by applications, the shares should be displayed as volumes in the same way as CD-Roms and such (it’s the case with Picard but I haven’t checked MP3Diags). When you access a Samba (and I assume other network filesystems like NFS) share, it’s mounted as a volume. ![]()
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